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تنزيل او تحميل كتاب Pharmaceutical microbiology برابط مباشر 

وصف الكتاب : Pharmaceutical microbiology من كتب علمية 

Cover for Pharmaceutical Microbiology 
Authors: 
B l Chaudhary 
Harshada jcshi 





... 
89 
3.5.1.2. Significance of Actinomycetes 
... 
90 
3.5.1.3. Classification 
... 
91 
3.5.1.3.1. Whole Cell Carbohydrate Patterns of Aerobic 
Actinomycetes 
... 
91 
3.5.1.3.2. Major Constituents of Cell Wall Types of 
Actinomycetes 
... 
91 
3.5.1.3.3. Groups of Actinomycetes Based on Whole 
Cell Carbohydrate Pattern and Cell 
Wall Type 
... 
92 
3.5.1.3.4. Actinomycetes with Multiocular Sporangia 
... 
92 
3.5.1.4. Actinomycetes and Related Organisms 
... 
93 
3.5.1.4.1. Group 
... 
93 
3.5.1.4.2. Genus 
... 
94 
3.5.1.4.3. Order 
... 
97 
3.5.1.4.4. Family 
... 
98 
3.5.2. Bacteria 
... 
102 
3.5.2.1. Salient Features 
... 
103 
3.5.2.2. Structure and Form of the Bacterial Cell 
... 
104 
3.5.2.2.1. Size and Shape 
... 
105 
3.5.2.2.2. Structure 
... 
105 
3.5.3. Rickettsia and Coxiella 
... 
107 
3.5.4. Spirochaetes 
... 
108 
4. Identification of Microorganisms 
... 
112 
4.1 Introduction 
... 
112 
4.2 Morphology 
... 
113 
4.3 Selective and Diagnostic Media 
... 
113 
4.3.1. Differential 
Media 
... 
116 
4.3.1.1. Eosin Methylene Blue Agar [EMB-Agar] 
... 
116 

xi 

4.3.1.2. MacConkey Agar 
... 
116 
4.3.1.3. Hektoen Enteric Agar [HE-Agar] 
... 
116 
4.3.2. Enrichment Media 
... 
116 
4.3.2.1. Blood Agar 
... 
116 
4.3.2.2. Chocolate Agar 
... 
117 
4.3.3. Characteristic Media 
... 
117 
4.3.3.1. Triple Sugar Iron Agar [TSI-Agar] 
... 
117 
4.4 Cultural Characteristics 
... 
119 
4.5 Biochemical Tests (or Properties) 
... 
120 
4.5.1. Carbohydrate (Sugar) Fermentation 
... 
120 
4.5.2. Litmus 
Milk 
... 
120 
4.5.3. Indole Production 
... 
120 
4.5.4. Methyl 
Red Test [MR-Test] 
... 
121 
4.5.5. Voges-Proskauer Test [VP-Test] 
... 
121 
4.5.6. Citrate Utilization 
... 
121 
4.5.7. Nitrate Reduction 
... 
122 
4.5.8. Ammonia Production 
... 
122 
4.5.9. Urease Test 
... 
122 
4.5.10. Production of Hydrogen Sulphide 
... 
123 
4.5.11. Reduction of Methylene Blue 
... 
123 
4.5.12. Production 
of Catalase [Tube Catalase Test] 
... 
123 
4.5.13. Oxidase Reaction 
... 
123 
4.5.14. 
Egg-Yolk Reaction 
... 
124 
4.5.15. Growth in Presence of Potassium Cyanide 
... 
124 
4.5.16. Composite Media 
... 
124 
4.6 Profile of Microbial Stains 
... 
127 
4.6.1. Preparation of Bacterial Specimens for Light Microscopy 
... 
128 
4.6.1.1. Standard Preparations 
... 
128 
4.6.1.2. Preparation of Smears for Staining 
... 
128 
4.6.1.3. Gram Staining 
... 
129 
4.6.1.4. Differential Staining 
... 
131 
4.6.1.4.1. Gram’s Stain 
... 
131 
4.6.1.4.2. Acid-Fast Stain 
... 
131 
4.6.1.5. Miscellaneous Staining 
... 
131 
4.6.1.5.1. Capsule Staining 
... 
132 
4.6.1.5.2. Endospore Staining 
... 
132 
4.6.1.5.3. Flagella Staining 
... 
133 

xii 

4.6.2. Microscopy : The Differential Instruments 
... 
133 
4.6.2.1. Concepts 
... 
133 
4.6.2.2. Microscope Variants 
... 
134 
4.6.2.2.1. Bright-Field Microscope 
... 
134 
4.6.2.2.2. Dark-Field Microscope 
... 
136 
4.6.2.2.3. Phase-Contrast Microscope 
... 
136 
4.6.2.2.4. Differential Interference Contrast 
(DIC) Microscope 
... 
139 
4.6.2.2.5. Fluorescence Microscope 
... 
139 
4.6.2.2.6. Electron Microscope 
... 
141 
4.6.2.2.6.1. Transmission Electron 
Microscope (TEM) 
... 
142 
4.6.2.2.6.2. Scanning Electron 
Microscope (SEM) 
... 
143 
5. Nutrition, Cultivation and Isolation : Bacteria-Actinomycetes-Fungi-Viruses
... 
146 
5.1 Introduction 
... 
146 
5.2 Bacteria 
... 
146 
5.2.1. Nutrition of Microorganisms 
... 
146 
5.2.2. Cultivation of Bacteria 
... 
147 
5.2.2.1. Binary Fission 
... 
148 
5.2.2.2. Normal Growth Curve of Microorganisms 
... 
149 
5.2.2.3. The Lag Phase of Microbial Growth 
... 
150 
5.2.2.4. Translational Periods Between Various Growth Phases 
... 
150 
5.2.2.5. Synchronous Growth 
... 
151 
5.2.2.6. Effect of Nutritional Concentration 
Vs 
Growth Rate of 
Bacterial Culture 
... 
152 
5.2.2.7. Growth Determining Techniques 
... 
152 
5.2.3. Isolation of Bacteria 
... 
154 
5.2.3.1. Selective and Diagnostic Media 
... 
154 
5.2.3.2. Bismuth Sulphate Agar 
... 
154 
5.2.3.3. Selective Media for Staphylococci 
... 
155 
5.3 Actinomycetes 
... 
155 
5.4 Fungi 
... 
156 
5.4.1. Reproduction of Fungi 
... 
158 
5.4.1.1. Asexual Reproduction 
... 
158 
5.4.1.2. Sexual Reproduction 
... 
159 
5.4.2. Industrial Importance of Fung 


Methods and specifications 
Testing of pharmaceutical products is carried out according to a Pharmacopeia of which there are a few types. For example: In America, the United States Pharmacopeia is used; in Japan there is the Japanese Pharmacopeia; in the United Kingdom there is the British Pharmacopoeia and in Europe the European Pharmacopeia. These contain a test method which is to be followed when testing, along with defined specifications for the amount of microorganisms allowed in a given amount of product. 

The specifications change depending on the product type and method in which it is introduced to the body. The pharmacopoeia also covers areas like sterility testing, endotoxin testing, the use of biological indicators, microbial limits testing and enumeration, and the testing of pharmaceutical grade water. 



عدد مرات التحميل : 19884 مرّة / مرات.

حجم الكتاب عند التحميل : 4.2 ميجا بايت .

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